Selection of E. coli Strains for Stable Transformation with Recombinant Plasmids Containing Full-Length Genome of Clinical HIV-1 Isolates
Using the E.coli strains for stable transformation with recombinant plasmids. Frequency of cell transformation with plasmids and elimination. Products of HIV-1 genome integration. Scheme of the cointegrate pPIC91 plasmid. Sequencing of the insert region.
Подобные документы
Исследование проявления водным экстрактом Acacia nilotica максимальной антибактериальной активности в отношении Е. coli. Особенность определения чувствительности микроорганизмов к антимикробному препарату и сравнительной антибактериальной активности.
статья, добавлен 17.08.2021Рemiclonal inheritance comprising the clonal transfer of one of the parental genomes to gametes - the main distinctive sign of green frogs. The length of erythrocytes dependence on body length for green frogs from the pond in the village Zhovtneve.
статья, добавлен 27.07.2016Розроблення нових методів виділення тирозил- і треоніл-тРНК синтетаз із T.thermophilus, виділення тирозил-тРНК синтетази із E.coli і характеристика чистоти одержаних препаратів білків. Визначення нуклеотидної послідовності тРНКTyr із T.thermophilus.
автореферат, добавлен 21.11.2013Stages of cultivation of multipotent stem cells derived from the neural crest in 3D collagen and fibrin hydrogels. Cell viability and growth. The conditions for the effective use of the results obtained for the development of the nervous system.
статья, добавлен 28.09.2016- 105. Plant Biochemistry
A leaf cell consists of metabolic compartments. The use of energy from sunlight by photosynthesis is the basis of life. Photosynthesis is an electron transport process. ATP is generated by photosynthesis. Mitochondria are the power station of the cell.
учебное пособие, добавлен 16.08.2012 Mutagenic potential of oxidized forms of nitrogen bases. The effectiveness of the inclusion of 8-oxo-DG in the growing DNA. The behavior of the molecule 8-oxo-DSTF in the migration trajectories. The transition of cells from normal state to cell death.
статья, добавлен 28.01.2016The physical, chemical traits of plant components used to impart functional properties to restructured meat products. An analysis of a number of sources of scientific literature has shown that for the manufacture of functional restructured meat products.
статья, добавлен 18.12.2023Study of consumer expectations of the properties of products for nutritional support of people with burn injuries. Forecasting the demand for these products among the target consumer segment. Analysis formation of rations for nutritional support.
статья, добавлен 28.08.2016Comparison of reproduction of second and fifth adenovirus serotypes (HAdV-C2 and -C5) in the various lines of lymphoblastoid cells. Intensive formation of infectious virus is similar in level to reproduction in permissive epithelial cells Hep-2.
статья, добавлен 14.10.2016A natural source of hyaluronic acid, humour rabbit vitreous. Regeneration by inhibition of cell differentiation. Morphology of rat bone marrow mesenchymal stem cells and NT2 cells. Expression of Oct4 and Sox2 genes. Cell surface antigen expression.
статья, добавлен 17.11.2012Assessment of biological diversity of the Lipetsk region. Determination of the number of nesting sites of rare bird species using cartographic data and remote sensing. Identification of the reasons for the variability of the landscape of the region.
статья, добавлен 18.05.2021Using the laws of physics and chemistry in the process of building cells. Technological gene information as instructions on the sequence of cell formation. Conditions for the functioning of information processes in living cells. Eternity of life cycle.
статья, добавлен 15.04.2024The discovery of new localities of nine species listed in the Red Book of Ukraine, and six regionally rare plant species during expeditionary research on the territory of Volyn region in 2013. The necessity for research phytodiversity Volyn region.
статья, добавлен 27.07.2016Nutritional environment: source of carbon, nitrogen. Inorganic salts, microelements and growth factors. Effects of oxygen, temperature. Anti-foaming, plasmid protection. Separation of cells, ion-exchange chromatography, hard forage additive l-lysin.
курсовая работа, добавлен 18.12.2021Conditional ideotype of the oilseed flax variety for the forest-steppe zone. Characteristics of the best breeding lines of oilseed flax and its elements in competitive variety testing. Characteristics of the best breeding lines by biochemical parameters.
статья, добавлен 30.06.2022The study highlights the issue of the state of productive capabilities of the local bream herd of the Dnieper-Buzka estuary region. The initial data for the performance of work tasks are the results of field research carried out during 2014-2018.
статья, добавлен 29.08.2022Research on health hazards of toxic fungal metabolites produced by Aspergillus fungi. Prevalence of aflatoxins in nature, contamination of human and animal food products. Food surveillance and aflatoxin standards provided in Mongolian National Standards.
статья, добавлен 12.12.2024Evaluation and analysis of relative degree of drought resistance by water content of tissue. Assessment of relative drought resistance by water-holding capacity of leaves genus momordica (cucurbitaceae) introduced under the conditions of Belgorod region.
статья, добавлен 29.11.2016Discussion the possibility of integrating methods of biotechnology, namely the elimination of oil pollution using biodegradation, obtaining of microbial nanoparticles, technology of microbial fuel cells and others. Bacterial growth on metal surface.
статья, добавлен 30.07.2016Wolbachiaspecies are obligate intracellular maternally inherited endosymbionts. Analysis of neurodegenerative phenotype of adult flies after bacterium elimination has shown decrease of its manifestations aftertetracycline treatment against Wolbachia.
статья, добавлен 27.07.2016- 121. Snow leopard
The snow leopard is a large predatory mammal from the cat family. The habitat, body structure and weight of the animal. Diet, life expectancy in the wild and captivity. The length of the jump when hunting. The main threat to the life of a predator.
презентация, добавлен 08.06.2020 Correlation of the properties of biofilms of microorganisms and malignant tumors indicates the existence of a certain analogy between them, which allows us to explain the essential circumstances. Intercellular substance plays a significant role.
статья, добавлен 18.09.2024The new insights into the process of interaction of T7-like bacteriophages FE44 and BA14 with lysogenic cells. The single and double lysogens possess Abi-phenotype regardless of genera and strain of bacteria that initially had normal phage sensitivity.
статья, добавлен 26.08.2016Test the hypothesis that pathogenesis induced in sensitive plants by ALS- and ACC-inhibiting herbicides is an active process which involves programmed cell death. A reliable marker of PCD is DNA fragmentation, which is carried out by endogenous nucleases.
статья, добавлен 12.06.2023The results of this study show changes which the egg of Puntius titteya undergoes. Oocyte sampled from the gonad. It has been concluded that such a structure can guarantee a full function during fertilization and during embryogenesis as a protection.
статья, добавлен 26.03.2020